General |
Detection modes |
UV-Vis absorbance
Fluorescence intensity
Luminescence
Fluorescence polarization
Time-resolved fluorescence |
Read methods |
Endpoint, kinetic, spectral scanning, well area scanning |
Microplate types |
Monochromator: 6- to 384-well plates
Filters: 6- to 1536-well plates
Imaging: 6- to 1536-well plates |
Other labware supported |
Microscope slides, Petri and cell culture dishes, cell culture flasks (T25)
Take3 Micro-Volume Plates |
Temperature control |
4-Zone incubation to 45 °C
with Condensation Control;
+0.2 °C at 37 °C |
Shaking |
Linear, orbital, double orbital |
Software |
Gen5™ Microplate Reader and Imager Software
Gen5 Secure for 21 CFR Part 11 compliance (option) |
Automation |
BioStack and 3rd party automation compatible |
CO2 and O2 control (option) |
Range: 0 - 20% (CO2); 1 - 19% (O2) Control Resolution:
+0.1% (CO2 and O2) Stability: +0.2% at 5% CO2; +0.2%
at 1% O2
Models for both CO2 and O2 or CO2 only are available |
Imaging System |
Imaging mode |
Fluorescence
Brightfield |
Imaging method |
Single color, multi-color, montage, time lapse, z-stacking |
Image processing |
Z-projection, digital phase contrast, stitching |
Camera |
16-bit gray scale, Sony CCD, 1.25 megapixel.
0.3 µm/pixel at 20x |
Objective capacity |
2 user-replaceable
objectives |
Objectives available |
1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x |
Image filter cube capacity |
4 user-replaceable fluorescence cubes plus brightfield channel |
Imaging filter cubes available |
DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7,Acridine Orange (ACR OR), CFP-YFP FRET, propidium,Iodide, chlorophyll, phycoerythrin, CY5.5, TagBFP |
Imaging LED cubes available |
365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm |
Automated functions |
User-trained autofocus, autofocus, autoexposure,
auto-LED intensity |
Autofocus method |
Image-based autofocus
User-trained autofocus
Laser autofocus (option) |
Positional controls |
Software control |
Image collection rate |
Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes
96 wells, 3 colors, 4x, 12 minutes
Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
96 wells, 3 colors, 4x, <7 minutes, 30 seconds |
Image Analysis Software option |
Gen5 Image+: Advanced image analysis
Gen5 Secure Image+: Advanced image analysis, 21 CFR Part 11 features |
Fluorescence Intensity |
Light source |
Xenon flash |
Detector |
PMT for monochromator system
PMT for filter system |
Wavelength selection |
Quad monochromators (top/bottom)
Filters (top) |
Wavelength range |
Monochromators: 250 - 700 nm (850 nm option)
Filters: 200 - 700 nm (850 nm option) |
Monochromator bandwidth |
Fixed, 16 nm |
Dynamic range |
5 decades |
Sensitivity |
Filters:
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate)
Quad Monochromator:
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom |
Reading speed (kinetic) |
96 wells: 11 seconds
384 wells: 22 seconds |
Luminescence |
Wavelength range |
300 - 700 nm |
Dynamic range |
>6 decades |
Sensitivity |
Monos: 20 amol ATP (flash)
Filters: 10 amol ATP (flash), 100 amol (glow) |
Fluorescence Polarization |
Light source |
Xenon flash |
Detector |
PMT |
Wavelength selection |
Filters |
Wavelength range |
280 - 700 nm (850 nm option) |
Sensitivity |
1.2 mP standard deviation at 1 nM fluorescein |
Time-Resolved Fluorescence |
Light source |
Xenon flash |
Detector |
PMT |
Wavelength selection |
Quad monochromators (secondary mode)
Filters (top) |
Wavelength range |
Filters: 200 - 700 nm (850 nm option) |
Sensitivity |
Filters: Europium 40 fM (4 amol/well, 384-well plate)
Monos: Europium 1200 fM (120 amol/well, 384-well plate) |
Absorbance |
Light source |
Xenon flash |
Detector |
photodiode |
Wavelength selection |
monochromator |
Wavelength range |
230 to 999 nm, in 1 nm increments |
Monochromator bandwidth |
4 nm (230-285 nm), 8 nm (>285 nm) |
Dynamic range |
0 - 4.0 OD |
Resolution |
0.0001 OD |
Pathlength correction |
yes |
Monochromator wavelength accuracy |
+2 nm |
Monochromator wavelength repeatability |
+0.2 nm |
OD accuracy |
<1% at 2.0 OD
<3% at 3.0 OD |
OD linearity |
<1% from 0 to 3.0 OD |
OD repeatability |
<0.5% at 2.0 OD |
Stray light |
0.03% at 230 nm |
Reading speed (kinetic) |
96 wells: 11 seconds
384 wells: 22 seconds |
Reagent Injectors (option) |
Supported detection modes |
All modes |
Number |
2 syringe pumps |
Supported labware |
6- to 384-well plates, Petri dishes |
Dead volume |
1.1 mL, 100 µL with back flush |
Dispense volume |
5 - 1000 µL in 1 nm increments |
Dispense accuracy |
+1 µL or 2% |
Dispense precision |
<2% at 50-200 µL |
Physical Characteristics |
Power |
250 Watts max. |
Dimensions |
20" D x 16.5" W x 17.5" H
(50.8 cm x 41.91 cm x 44.5 cm) |
Weight |
80 lbs (36.3 Kg) |
Regulatory |
Regulatory |
CE and TUV marked. RoHS Compliant. Models for In Vitro
Diagnostic use are available. |